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Thanks. It is not publicly available, this is my own making, in a laboratory. And yes, it is an intratympanic injection, injected cold, it gels when it reaches body temperature, hence the slow release. Modified FX-322 was not a miracle, maybe slight benefit to one ultra high frequency, also...

Nothing new. I'll be doing some other stuff, I have already done progenitor cell activation (adapted Frequency Therapeutics intratympanic formulation, slow release BDNF coming soon).

I had an ENT suction out lidocaine before an IT shot. It was not that loud, personally.

Yep, tastes even worse than HPN-07.

They saw audiogram improvements in their animal models.

The exosomes that I receive are derived from umbilical cord mesenchymal stem cells. I can get the cells themselves, too, but cell therapy is riskier (and the cells will likely not cross the round window if injected intratympanically). I'll reiterate the same thing I have posted before...

Could you please provide any publications on intratympanic exosomes then? I'm talking human trials, I do know they seem to be effective in animals. However, hearing-wise, they did nothing to me (systemically at very high doses). I will try growth factors (BDNF and/or NT-3) intratympanically...

Progenitor Cell Activation, what Frequency Therapeutics were doing.

I personally know two people who have had stem cell exosomes administered intratympanically (1 week of IT shots, a shot every other day, so either 3 or 4 shots, I forget). No improvement. One shot was around 0.5 ml, containing 50-100 billion exosomes. All that aside, the PCA approach worked...

Literally their patents.

For those interested, Spiral Therapeutics has patents on their slow release gel. It's a chemically crosslinked polymer - you mix PEG esters with trilysine amines and can control the crosslinking reaction by adjusting the pH. This gel is more durable than a P407 gel (Otonomy and Frequency...

Yes, and from this and their modelling they 100% knew that it would not really be effective below 9-10 kHz.

To be honest, FX-322 did reach 10 kHz at required concentrations of CHIR99021 and sodium valproate to work. Now testing speech was an interesting choice, but it makes sense because understanding speech is one the main goals of hearing restoration. Getting deeper required *some* changes to the...

Headphones, I could not hear the sound for some reason. Increased the volume, still could not hear it. But it did damage.

It was not. The regular FX-322 is 88 mg/ml sodium valproate (HDACi), 3.1 mg/ml CHIR99021 (GSK3i), 5% w/v DMSO (solvent for CHIR) and 16.1% w/v Pluronic P407 (gelling agent). FX-345 is likely (based on very limited info they released) the same 88 mg/ml sodium valproate, 5% w/v DMSO and 16.1% w/v...

It was just high frequency noise really, coming from either the motor or the inverter. I am not sensitive to that anymore.

I 100% know I can get it done. :-)

I will *maybe* have very high dose exosomes IT in the near future, we will see. 200b/ml concentration.

Not worth it, as the number of exosomes is low for the price. Even with really high doses IV (not their exosomes), I saw no improvement in hearing or tinnitus, but my hyperacusis is gone. Unsure if that was natural or that exosomes helped.

5 months ago, no effect apart from quickly fixing mild ETD I had due to an infection.

I have tried intranasal exosomes, 100 billion per day for a week. Outer ear will likely do nothing. Intratympanic is where it's at!

I'm in my early 30s. I haven't looked into 'equivalent dosing', but 20 billion exosomes is low.

This is exactly my point. Unsure if I can disclose that, to be honest. We will see.

Visual snow is the same as it is likely directly correlated with the signal loss due to hearing loss. Again, I cannot recommend this treatment because of the decently high cost, the low absolute numbers of exosomes and the lack of evidence of efficacy.

Hyperacusis - after Anova (but I think it was getting better on its own), and I had one migraine after Anova, and none after the other exosomes. I still take them once every few weeks, basically whenever I want.

Well I have had no improvement in hearing, but I haven't tried intratympanic things yet. I will very soon, though :-)

The trial with children was with stem cells themselves, if I recall correctly.

Sorry, I am under an NDA and cannot disclose these things. For what it is worth, I do not think Anova's low doses would help much.

I do not consider distortions part of hyperacusis at this point. Yes, I have hearing loss that presents as distortions.

Exosomes have completely eliminated my migraines (which had never been an issue, but still).

It was both. At this point I would not recommend it, I think one needs to have a decently high concentration of growth factors and/or exosomes on the round window for this kind of stuff to be effective.

Hi, Still nothing. But I am hyperacusis-free, so that is good. I am continuing with intravenous umbilical cord stem cell exosomes, at very high doses (2 trillions every few weeks, compared to around 20 billion at the clinic). But so far nothing. The next step would be intratympanic things. :-)

My hearing loss and tinnitus are the same, and I am still pain and pressure-free. The problem is that I cannot say whether the exosomes actually helped or whether it was simply time.

Has anyone asked if the IV dosage is different from the oral dosage?

Steroids right after the onset + NHPN-1010 work as a 'quick fix' in some cases.

First of all, this was stem cell exosomes, not the cells themselves. Second, there is some pre-clinical data that they are effective, but one needs higher doses. In principle, I could try intratympanic exosomes, too. I do have hearing loss, just not something that's visible on an audiogram (a...

My 'reactivity' is/was slightly different. After sound exposure, my tinnitus sounds are louder for a day or two. It's lessened a little bit. Could have been just time really. Hard to say.

I have no other conditions, except maybe some red spots on my face. The exosomes cleared it up a bit. Just to reiterate, in my opinion, their dosage is way too low for the price you pay. In addition, one should be absolutely sure they do not have cancer as exosomes in some instances may speed...

It was mild to moderate, tops. I would get ear pressure after noise exposure like road noise or talking to people on Zoom. If I continued, I would get deep stabbing pain going all the way to my throat. I also had loudness hyperacusis where some high frequency sounds would be amplified, it's...

Hi, Life is okay-ish. I have no sound sensitivity, as mentioned, so I wear headphones daily with no downsides. Yesterday I went out to eat and the noise in the restaurant was peaking at 80 dB. I did not wear plugs, even though I did have them with me, just in case. Nothing happened. Tinnitus...

Looking back, nope. It was noise. Vaccinated people usually have lower viral loads, so yes. Vaccine 'risks' are way blown out of proportion by people who read 'alternative news' sources.

I got another Pfizer booster and I am completely fine. I had a sore arm for one day, that was it. Tinnitus is quiet. Get vaccinated because COVID-19 DOES hurt the auditory system. With the rate that Omicron is spreading, most people will have some contact with the virus, unless they can isolate...

No, sound distortions are the same, unfortunately. I am less sensitive to sound in general, however. Difficult to say if it was the exosomes, but I definitely have no ear pressure anymore.

I had ear pressure after exposure to sound, and if I continued exposing myself to sound, it would become painful - pain deep inside, sort of going into my throat. It is better now. But again I cannot say whether this is due to the exosomes, or simply time. Maybe, I am waiting, of course...

I no longer get ear pressure/pain from medium intensity sound, but I haven't tried exposing myself to very loud sound (85 dB+ for extended periods). My tinnitus still increases after moderate sound exposure and goes down in a day or so. I have no other health problems except for mild visual...

My update: It is now roughly 4 weeks past my last IV. I tried to log my symptoms, but live life normally otherwise. I have noticed that in about a week after the last IV, my ear pressure started to go down. Before I would get ear pressure in response to sound exposure, but not anymore, or not...

Yes, the idea is the same, but it's going to be better.

I am not the forum's favourite hydrocarbon, but I can try to answer this! Their new GSK3i is supposedly more potent (replacing CHIR99021) and they're possibly using a higher concentration of Valproic Acid, courtesy of using a purified Poloxamer P407 (unpurified P407 will not allow for higher...

In the cochlea. Check this paper out: Human mesenchymal stromal cell therapy for damaged cochlea repair in nod-scid mice deafened with kanamycin

I personally would not do stem cells, only exosomes/secretome. Simply because the cells will only get to your cochlea in small numbers and they are unlikely to engraft there. And also the added danger of injecting live cells (especially allogeneic) vs injecting small extracellular vesicles.

You are misunderstanding me, it's not about oxygen intake, it's because you are in an airtight capsule while you undergo the procedure and they increase the air pressure there; if you can't equilibrate pressures, you might get barotrauma.

The vented plugs have a hole for the air to escape, thus making it easy to equilibrate the pressure between the outer ear and the middle ear. I used my musician's plugs.

This only means the procedure is pretty safe really.

Earmuffs will make it harder for you to equilibrate pressures. Only use vented plugs and that's it.

The main compound is not the same, though, is it? It's CHIR99021 in FX-322 vs (possibly modified) LY2090314 in FX-345.

I feel fine, I've had 3 IVs, and I can schedule more, but I want to wait for results first. I will give an update in a few weeks. Some positive things are happening, but nothing miraculous.

I am saying that phones/Bluetooth connection will not unwind the DNA because of 1) too little energy emitted by those devices, and 2) too little energy absorbed by your body, only causing an insignificant heating effect. The DNA binding/unbinding is a stochastic process really and happens all...

This, I would like to add that unwinding a double-stranded DNA helix takes a lot of energy. Even if one base-pair disconnects due to thermal energy, it will reconnect because the others are still holding the helix together. For a 12 base pair DNA strand (very short!) to completely unwind, it...

There are other, and, in my opinion, safer things to try before going there. I have now had my first IV, two more to go. I don't expect any results sooner than a few weeks from now.