DIY Cord Blood Stem Cell Treatment

[Steve Tinnitus]

Current cord blood stem cell treatments to regenerate your hearing cost $22000+ in Thailand or Korea.

I found that you can actually buy cord blood online from:
Buy Cord Blood Here < link
Its $95 for only 1ml, but this got me thinking...

Here is the machine to separate stem cells from cord blood:
AXP stem cell machine from Cesca therapeutics < link

Who's to say the average Joe couldn't do the following:
Step 1: Persuade a pregnant lady or naughty midwife to sell otherwise useless cord blood to you. - Easy to do
Step 2: Have the blood screened to check if its clean / matching blood type. - Easy to do
Step 3: Separate the stem cells yourself. - Easy to do
Step 4: Inject them yourself. - Easy to do

You could do the treatment yourself 10 times over! regenerate your hearing and be tinnitus free as well as making you look younger so you will be extra sexy too.

I am aware that this is a completely crazy idea and there is lots of other points that I would like to mention, but for now I would like to welcome thoughts or suggestions about this.

Thanks in advance,

Steve.

 

[Rubenslash]

Any scientific evidence that this can regenerate your hearing?

 

[Ambassador]

Inject them where?

 

[Steve Tinnitus]

To get the ball rollin'

Mesenchymal Stem Cell Isolation from Cord Blood
1. Dilute cord blood with RPMI Medium 1640 with a 3:1 ratio (3 parts cord blood to one part RPMI).
2. Isolate mononuclear cells (MNCs) by density gradient centrifugation at 400xg for 30 minutes at room temperature using Ficoll-Paque™ Premium according to the manufacturer's instructions.
3. Transfer MNCs to new centrifuge tube and add PBS with a 1:3 ratio (1 part MNCs to 3 parts PBS).
4. Centrifuge at 400xg for 10 minutes at room temperature.
5. Remove supernatant and resuspend cells by adding culture medium and plate.
6. Incubate at 37°C, 5% CO2 incubator overnight.

Just to mention, there are various price ranges of blood centrifuge machines (even under $80) on eBay shipped worldwide.

RPMI 1640 and PBS available at thermofisher.com

Now go find some preggos.

Steve

 

[The Red Viper]

The real question is whether the mesenchymal stem cells will differentiate into whatever cells are needed to repair tinnitus. The deeper question is whether it's the dead nerve cells or the dead hair cells that cause tinnitus (or both). I see no problem with this experiment, but you should look into the scientists that have grown cochlear cells in a dish (google "ear in a dish"). I would first try to grow a cochlea, damage it, and then inject the mesenchymal stem cells.

 

[linearb]

this is insane and dangerous, full stop

 

[jdm]

I disagree and view this as potentially ground breaking for affordable DIY biohacking. Please keep us updated.

 

[Oceans63]

This sounds really interesting! What else have you learned about doing this yourself? What equipment and materials are needed?

 

[ifilip]

I found that you can actually buy cord blood online from:
Buy Cord Blood Here < link
Its $95 for only 1ml, but this got me thinking...

Are you kidding? Buying cord blood online. How can you be sure if it's even real cord blood?
It's like buying those penis enlargement pills.

Who's to say the average Joe couldn't do the following:
Step 1: Persuade a pregnant lady or naughty midwife to sell otherwise useless cord blood to you. - Easy to do
Step 2: Have the blood screened to check if its clean / matching blood type. - Easy to do
Step 3: Separate the stem cells yourself. - Easy to do
Step 4: Inject them yourself. - Easy to do

Easy to do? Well if you have a home laboratory and are skilled biologist/physician etc., then maybe yes.

 

[CricketEars]

I read this article a while back where a clinic just randomly injected stem cells into elderly women's eyes and it did irreparable damage. Be careful!

https://www.cbsnews.com/news/stem-cell-treatment-blinded-three-women-florida/

 

[Everyloop]

I'm fairly certain it has to be your own cord blood in order for it to be effective.

 

[Nick07]

The fact that this takes place in Thailand means it's not reputable. Secondly as I understand it embryonic stems taken from a foreign source tend to form tumors. Thirdly as I understand it every time scientists have tried to induce cells into becoming the hair cells needed they have to be extremely young and manipulated, and the results have been low even with those. More over if the goal is the elimination of tinnitus there's no guarantee it would work even if a marginal amount of hearing was restored.

Another thread on treatments mentioned detecting nerve damage and applying a nerve numbing agent to the damaged areas to give tinnitus relief. If you instead were able use polypotent adult stem cells to repair the damage you could eliminate tinnitus. There's currently trials going involving treatments for joint and bone disorders, but in the article I read it was suggested they could be used for anything. My guess is they're missing something fundamental for things like hearing restoration and spinal cord injuries. Otherwise they feel like they want to do something they know will be successful.

 

[Steve Tinnitus]

I have actually given up on everything and have come to terms with the screaming in my head for now,
hence I have been avoiding this website recently.

The original post topic was just a speculation as to what could be possible for an average person to achieve.

However, I'm going to reply to a few questions because I don't have a life...

I'm fairly certain it has to be your own cord blood in order for it to be effective.

Not true, it is possible for donor cord cells to be effective.
Outcomes are improved when the cells for transplant closely match the patient. However, studies show that cord blood does not need to match as closely as bone marrow or peripheral blood for a successful transplant.
(I found a good article on the process for matching cord cells but I can't remember it, apologies)

I read this article a while back where a clinic just randomly injected stem cells into elderly women's eyes and it did irreparable damage. Be careful!
https://www.cbsnews.com/news/stem-cell-treatment-blinded-three-women-florida/

Please don't inject stem cells into your eyes.
I can't take anything seriously from this article as CBS is a fake American news outlet.
Just to note: it also says "Japanese researchers treated a woman with the same eye disease using stem cells. One year after surgery, her vision had stabilized and there was no sign of lasting side effects"

The fact that this takes place in Thailand means it's not reputable. Secondly as I understand it embryonic stems taken from a foreign source tend to form tumors. Thirdly as I understand it every time scientists have tried to induce cells into becoming the hair cells needed they have to be extremely young and manipulated, and the results have been low even with those. More over if the goal is the elimination of tinnitus there's no guarantee it would work even if a marginal amount of hearing was restored.

Cord blood stem cells, not embryo.
I still agree this could potentially have side affects, but I also believe it really holds potential to resolve hearing loss and tinnitus

The reason I started thinking about of this was because my friend is a midwife and could collect endless supplies of cord blood samples. I also have a couple of really bright Biologist friends, their work does not actually specialise in human sciences but from chatting to them I'm sure they have a greater knowledge about all of this than myself.

I was also recently talking to a friend about a local musician that recently killed himself because of their struggle with tinnitus. This is obviously a condition that severely affects peoples life, in some cases there are people who would be willing to risk everything for anything.

If you think this is a stupid idea then stick to Chinese herbs and sipping CBD oil and wasting your time.
I admit I don't take this serious and I am aware it is a crazy concept. But please feel free to post if you have a serious suggestion. There are people at the end of the line that could be inspired to find a feasible treatment themselves.

Godspeed

 

[Aaron123]

I can't take anything seriously from this article as CBS is a fake American news outlet.

not sure why you would think that. Hopefully the NEJM isn't fake news: http://www.nejm.org/doi/full/10.1056/NEJMoa1609583#t=article

The Japanese case is completely different. You should read the entire paragraph in the CBS article as it notes the differences between the stem cell clinics and the serious research in terms of "care and prudence". Alternatively, you can read about the Japanese patient here: http://www.nejm.org/doi/full/10.1056/NEJMoa1608368#t=article. They paused after one patient and resumed this year using allogeneic rather than autologous stem cells. You can read more about that here: https://www.nature.com/news/japanes...rammed-stem-cells-from-another-person-1.21730

This is a big deal as these are, I believe, the first recipients of iPSCs. As far as I know though, this is nothing to do with cord blood.

 

[ifilip]

I read this article a while back where a clinic just randomly injected stem cells into elderly women's eyes and it did irreparable damage. Be careful!

https://www.cbsnews.com/news/stem-cell-treatment-blinded-three-women-florida/

The problem is that dishonest clinics hurry with treatment when it has not been tested properly yet. They think that if they just inject stem cells into the body it will do miracles. But stem cells are not that easy. I believe they will treat most conditions, but not like that. The procedure will be more complicated than just "throw stem cells at the patient" like these clinics do.

 

[lymebite]

This DIY approach may not be so far fetched as it sounds. Adipose cells from liposuction is rather complicated yes, but umbilical cord stem cells are a different story - they are ordered from a factory which prepares them, ships them in a vial, and then they are simply injected into the patient with an IV. Not quite as simple as self-administering an insulin shot or a B-12 shot, but maybe not so far off from that.

Here is a stem cell clinic located in Indiana, USA that offers umbilical cord mesenchymal stem cells. Sure, they inject them for you, but it looks like a very simple procedure:

http://www.regenmedky.com/umbilical-cord-stem-cell-therapy/

Effectiveness for tinnitus? who knows, it will take a lot more people from TTalk to try and report back. But simplicity - it does seem very simple.

Here is an article that touches on the simplicity:

"How is it administered? The most common method is injection. Until now, this has been a fairly complicated process to achieve desired results. While your doctor may just be "perfecting" their stem cell routine, they are quite likely using already outdated technology. This is what you need to know. An injectable is currently available that is showing an extremely high success rate. It costs less than most PRP protocols, requires only one injection, with a smaller needle than PRP, during a doctor's appointment that will take about 20 minutes. One and done! Orthopedic doctors, podiatrists, periodontists, and dermatologists, are most likely to embrace this technology."

http://www.societywellness.com/stem-cell-technology-part-two-umbilical-cord-blood/